This video shows the parts of the pump and describes how the pump operates to enable continuous delivery osmotic pump mechanism on vimeo. The first step in formulating a test solution to be administered is to decide on the dose or hourly mass flow of test material you wish to deliver. Myelin basic protein regulates primitive and definitive neural stem cell proliferation from the adult spinal cord. Alzet osmotic pumps for laboratory animal dosing overview. These determinants are the level of drug exposure and its duration, and the spatial distribution of drug relative to the target tissue. The video also describes proper filling of the alzet pump and and surgical techniques for implantation in rats and mice. Osmotic pumps are miniature, infusion pumps which provide researchers with a safe method to deliver agent in vivo. Durect corporation alzet osmotic no 1004 pump alzet. This 20 minute video describes the alzet pump mechanism, and the advantages of continuous infusion over frequent injections.
For nearly 30 years, these pumps have been the recognized standard for infusion in unrestrained laboratory animals, including nude or intraperitoneally, these miniature infusion pumps. Preparation and implantation alzet osmotic pumps sop9 osmotic pumps manufactured by alza corporation palo alto, ca are designed for constant rate delivery of test solutions over a. Isoproterenol concentration varies depending on the body. Alzet pumps have been exposed to a sterilizing dose of radiation from a 60co source. Alzet osmotic pumps, can effectively induce a peripheral antihyperalgesic effect. Jose currently holds the position of senior product marketing manager for alzet pumps and ancillary products at durect corporation. Microosmotic pumps for continuous release of the tyrosine. Implantable mini osmotic pump implantable mini osmotic pump is made up of three concentric layers, drug reservoir, the osmotic sleeves and the rate controlling semipermiable membrane.
Individual lots of pumps may vary from this target within limits. This video shows the parts of the pump and describes how the pump operates to enable continuous delivery alzet resource videos osmotic pump mechanism on vimeo. Alzet pumps operate because of an osmotic pressure difference between a compartment within the pump, called the salt sleeve, and the tissue environment in which the pump is implanted. Alzet osmotic pumps have been a part of successful research for over 40 years. Jose gadea has over 15 years of technical and marketing experience working with alzet osmotic pumps.
Implantation of miniosmotic pumps and delivery of tract. Special delivery highlights the value of alzet pumps in select research applications, including diabetes and obesity therapy with. Osmotic drug delivery system as a part of modified release dosage form. Osmotic pumps are miniature infusion pumps for the continuous dosing of laboratory animals. Alzet micro osmotic pump model 1004 performance of a pump if they accumulate on its surface. Osmotic pump of this form are available under trade name alzet. We are currently working remotely for order processing while maintaining shipping operations. Technical information manual appended pump specifications. For over 40 years, alzet osmotic pumps have been used in cancer studies as a reliable and convenient method for continuous dosing of unrestrained lab animals.
Gradient generation by an osmotic pump and the behavior of human mesenchymal stem cells under the fetal bovine serum concentration gradient article pdf available in lab on a chip 712. Osmotic pumps as drug delivery system by dana smadi on prezi. Isoproterenol 17 mgkgday was administered to mice using alzet osmotic minipumps durect corp, cupertino, ca. The pump reservoir cannot be filled to less than capacity, since doing so would result in a reservoir filled partially with solution and partially with air, causing discontinuous delivery of the solution. Alzet mini osmotic pump model 2001 instruction and specification sheet durect corporation manufactures a miniature implantable pump for use in laboratory animals. The elementary osmotic pump is a new delivery system for drugs or other active agents. Osmotic pumps of different sizes and drug duration are available for purchase from alzet. Watch this short video for an overview of the alzet technology, benefits and applications. Osmotic pumps release drug at a rate that is independent of the ph and.
Development and evaluation of controlled porosity osmotic. Pdf osmotic micropumps for drug delivery researchgate. Graphical illustration displaying the temperature dependence of the delivery rate of alzet miniosmotic pumps. Alzet pumps are drug discovery tools that offer researchers enhanced control over test compound levels in plasma and tissues. The alzet mini osmotic pump model 2001 delivers solutions continuously for 7 days without the need for external connections or frequent handling of animals. Development and evaluation of controlled porosity osmotic pump for nifedipine and metoprolol combination rajagopal kumaravelrajan, 1 nallaperumal narayanan, 2 and venkatesan suba 3 1 department of pharmaceutics, c. Continuous delivery ensures constant compound levels in plasma or tissues for maximised therapeutic efficacy and reduced adverse effects. Osmotic drug delivery system as a part of modified release. Page 3 page 4 page 5 effective therapeutic dosing methods for.
These small, implantable pumps deliver solutions at controlled rates over prolonged periods, enabling compound levels to be maintained within their therapeutic range to maximize. Chemical compatibility test kit 0004750 for alzet osmotic pumps. As the tablet passes through the body, water is absorbed through the semipermeable membrane via osmosis, and the resulting osmotic pressure is used to push the. It is generally used intravenously, although subcutaneous, arterial and epidural infusions are occasionally used infusion pumps can administer fluids in ways that would be impractically expensive or unreliable if performed manually by nursing staff. Alzet pumps are not compatible with aliphatic and aromatic hydrocarbons, such as heptane, toluene, and natural oils. Alzet pumps have been used to target delivery to a wide variety of sites including the spinal cord, spleen, liver, organ or tissue transplants, and wound healing sites. We chose the alzet model 1004 minipumps with a 100.
Department of pharmaceutics, institute of pharmacy, nirma university, sarkhejgandhinagar highway, ahmadabad 382 481, gujarat, india. Demonstrated procedures include subcutaneous and interperitoneal implantation, and the use of a catheter for intravenous jugular, intrathecal, intragastric, and cranialcerebral infusion. Convenient, reliable, and costeffective alternative to chronic injections. From the slideshare landing page click the download button to retrieve the file. Osmoticcontrolled release oral delivery system wikipedia. Louis, mo and is dissolved in phosphate buffered saline. Empty reservoir within the core of the pump is filled with the drug or hormone solution to be delivered and is surrounded by salt chamber with impermeable layer between them it is an implantable osmotic pumps for laboratory animal the pump are used to deliver homogenous solution or suspension continuously at a. Small infusion pumps and alzet catheters for continuous dosing of unrestrained laboratory animals. After filling the pump body with angii, the flow moderator is inserted to seal the pump.
These infusion pumps continuously deliver drugs, hormones, and other test agents at controlled rates from one day to six weeks without the need for external connections or frequent handling. Osmotic minipumps animal considerations osmotic minipumps can be implanted subcutaneously or intraperitoneally in rodents following the guidelines in table 5. By varying the concentration of the active agent in the dispersion, the amounts delivered can be varied at a constant temperature amkraut et al. Prior to inserting the minipumps, mice will be anesthetized using avertin 0. This article introduces a standard protocol used to induce aortic aneurysms via subcutaneous infusion of angiotensin ii angii from implanted osmotic pumps. The osmotic controlled release oral delivery system oros is an advanced controlled release oral drug delivery system in the form of a rigid tablet with a semipermeable outer membrane and one or more small laser drilled holes in it.
Alzet osmotic pump, as indicated by its name, was a system developed by alza corporation for experimental research. Osmotic pressure is the hydrostatic pressure produced by a solution in a space divided by a differentially. The pumps functionthrough an osmotic gradient between the pump and the tissue where the pump is implanted. Osmotic, osmosis delivery, pump, solvent, alzet, telescopic. The historical development of osmotic systems includes development of the rosenelson pump, the higuchileeper pumps, the alzet and osmet systems, the elementary osmotic pump, and the pushpull system. Isoproterenolinduced heart failure mouse model using. Implantation of osmotic pumps in rodents university of. Alzet osmotic pumps are cylindrically shaped see fig. Premature excess release from the alzet osmotic pump. The alzet pump is easily adapted for delivery of smaller volumes by attaching a small external reservoir made of catheter tubing.
Alzet pumps can also be used for targeted delivery, where the effects of a drug or test agent are localized in a particular tissue or organ, by means of a catheter. Durect corporation alzet osmotic pump model 1002 manufacturer. A filling needle is included in the box with osmotic pumps. Evaluatio of an osmotic pump for microdialysis sampling in. This document is intended to serve as a guide to help standardize the procedure by which an. Mini osmotic pumps alzet 2001, 2004, 2006 or micro osmotic pumps alzet 1004. Alzet pumps deliver by the process of osmotic displacement.
This video describes the inner parts of an alzet pump and how they operate to enable continuous delivery of experimental agents. Veterinary bioscience institute alzet osmotic pumps. For the rest of spring semester, boston university has directed undergraduate students to return home, canceled inperson classes, moved to remote teaching, called off all events and athletics, and minimized lab research. Plastic test tubes for dissolution of the reagent or incubation of osmotic pumps 4. We recommend use of the smallest size pump allowable for the solubility of the agent and experimental duration. Empty reservoir within the core of the pump is filled with the drug or hormone solution to be delivered and is surrounded by salt chamber with impermeable layer between them it is an implantable osmotic pumps for laboratory animal the pump are used to. Prior to durect, jose worked as a therapeutic sales specialist for alza pharmaceuticals. Alzet surgical implantation procedures video on vimeo.
In a study published in the british journal of pharmacology, lever et al. In wake of the covid19 virus, braintree scientific, inc. Alzet osmotic pumps are a simple, convenient and reliable method for continuous dosing of laboratory animals, including mice and rats. Alzet pumps have been used to target delivery to a wide variety of sites including the spinal cord, spleen, liver, organ or tissue transplants, and wound. Rodent restrainers transport cages handling gloves holding cage handling equipment.
Osmotic drug delivery has come a long way since australian physiologists rose and nelson developed an implantable pump in 1955. Introduction osmosis refers to the process of movement of solvent from lower concentration of solute towards higher concentration of solute across a semi permeable membrane till the equilibrium achieved. Contact with such substances can result in a pump malfunction. These minipumps provide researchers with a method for controlled and continuous agent delivery in vivo. Alzet osmotic pumps are small, implantable pumps for research in mice, rats, and other laboratory animals. An infusion pump infuses fluids, medication or nutrients into a patients circulatory system. Osmotic micropumps for drug delivery are applied to a broad. Preparation and implantation of alzet pumps saha cardiovascular. Osmotic pumps consist of an inner core containing drug and osmogens, coated with a semipermeable membrane. These infusion pumps continuously deliver drugs, hormones, and other test agents at controlled rates from one day to six weeks without the need for external connec. Alzet osmotic pumps implantable infusion pumps for. Channel is long and straight electro double layer has a finite width cross section of the channel is constant along the flow direction the applied electric field is uniform and along the x axis of the channel the potential at the wall is constant and. Through continuous infusion, these pumps maintain a welldefined, consistent pattern of drug exposure throughout the duration of the experiment.
Microglia and brain angiotensin type 1 receptors are involved in desensitising baroreflex by intracerebroventricular hypertonic saline in male spraguedawley rats. Pdf gradient generation by an osmotic pump and the. This video describes the inner parts of an alzet pump and how they operate to enable continuous delivery of. Antifreeflow devices prevent blood from draining from the patient, or infusate from freely entering the patient, when the infusion pump is being set up. Batteries, so the pump can operate if the power fails or is unplugged. Osmotic pump delivery system authorstream presentation. Alzet pumps may also be connected to a catheter to deliver the pump contents directly into the venous or arterial systems, the brain, or into any organ or tissue. For instructions on how to prepare the pump for these applications, refer to section vll. Only the magnitude and the duration of delivery change. Sep 16, 2015 alzet pumps deliver by the process of osmotic displacement.
As the core absorbs water, it expands in volume, which pushes the drug solution out through the delivery ports. We understand the importance of your studies and will do our best to fulfill your orders in a timely manner. This video provides an overview of the alzet technology, benefits, and applications. Durect corporation alzet osmotic pump model 1002 alzet. Rose nelson pump the first osmotic pump developed in 1955 for the delivery of drugs to the sheep and cattle gut composed of three chambers water to be loaded prior to use was the drawbacks of rose nelson osmotic pump water chamber rigid semi permeable membrane elastic diaphragm delivery orifice drug chambersalt chamber 15. These minipumps deliver drugs, hormones, and other test agents at continuous and controlled rates, for durations ranging from one day to six weeks, without the need for external connections or frequent handling. Alzet pump information is now available via the internet. Osmotic drug delivery uses the osmotic pressure for controlled delivery of drugs by using osmogens for upto 10 16 hrs. Hidden camera investigation on what really happens to your car cbc marketplace duration. Durect corporation alzet osmotic no 1004 pump alzet osmotic. Alzet pumps allow researchers to understand and optimize the key determinants of drug action. Pdf osmotically controlling drug delivery system is one of the best method of.
The historical development of osmotic systems includes development of the rosenelson pump, the higuchileeper pumps, the alzet and osmet systems, the elementary osmotic pump, and the pushpull. In this research, rats were implanted with 7day minipumps model 2001 containing 24 mgkgday pyridostigmine, and blood samples were taken at intervals from 5 to 60 min after implantation. Alzet osmotic pumps are small, selfpowered, infusion pumps for. A series of sampling frequencies starting with 90 min and decreasing to 10 min using an osmotic pump same type, alzet 2ml1. The nominal performance is a target for all pumps manufactured. Also the flow moderator is inserted into the body of.
Alzet mini osmotic pumps of desired delivery period 1, 3, 7, 14 or 28 days for mice durect corporation, cupertino, ca. Osmotic controlledrelease oral delivery system an overview. Rose nelson pump the pump pushpull osmotic pump advantage rose nelson pump was the first pump produced it was composed of three chambers. A down pressure sensor will detect when the patients vein is blocked, or the line to the patient is kinked. To cut the mass flow rate in half, use half the concentration.
Apr 17, 2018 oral osmotic drug delivery system classification of osmotic dds 8. Miniosmotic pump infusion model to investigate the systemic. Solvent and agent compatibility with alzet osmotic pumps bases, dilute or low concentrations of dmso and alcohol, and up to 100% propylene and polyethylene glycol. By attaching the proper catheter, alzet osmotic pumps can deliver substances into the venous or arterial circulation, into the brain, or into any organ, lumen or solid tissue. Preparation of alzet osmotic pumps for subcutaneous implantation reagentssupplies 1.
Osmotic pumps are most promising systems for controlled drug delivery. Alzet pumps are a simple, convenient and reliable method for continuous dosing of laboratory animals, including mice and rats. Isoproterenol 17 mgkgday was administered to mice using. Sep 28, 2015 osmotic pumps continuously deliver compounds at a constant rate into small animals. Alzet resource videos osmotic pump mechanism on vimeo. Weigh out the calculated amount of angii use a sterile plastic tube. Recent advances include development of the controlled porosity osmotic pump, and systems based on asymmetric membranes.
Alzet pumps can be implanted subcutaneously or intraperitoneally following the animal size guidelines below. Alzet osmotic pumps can be implanted subcutaneously or intraperitoneally following the animal size guidelines. This paper alerts investigators to the possibility of an excessive early release from the alzet osmotic pump. Use the forceps to open the space under the skin in the back of the animal where the pump will be implanted. The invention of alzet osmotic pumps in the 1970s allowed. Pdf osmotic drug delivery system as a part of modified. Osmotic pumps can be used for systemic administration. This is a pdf file of an unedited manuscript that has been accepted for publication. Osmotic pumps release drug at a rate that is independent of the ph and hydrodynamics of the dissolution medium.